rabbit anti-mouse lyve-1 polyclonal antibody  (Servicebio Inc)

Journal: International Journal of Molecular Sciences

Article Title: Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice

doi: 10.3390/ijms21113885

Figure Lengend Snippet: Number and exclusive area of lymphatic vessels with lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) immunoreactivity. ( A ) Representative images of immunohistochemistry using anti-LYVE-1 antibody. Scale bars (magnification): 50 µm (200×). ( B ) Number of lymphatic vessels in high power field (HPF) (mean ± SE). (C) Exclusive area of lymphatic vessels in HPF (mean% ± SE). In (B) and ( C ), results of multiple comparisons within the same day groups are indicated. ** p < 0.01: significantly different between X-ray/ADSC (−/−) and X-ray/ADSC (+/−). †† p < 0.01: significantly different between X-ray/ADSC (−/−) and X-ray/ADSC (+/+). ‡ p < 0.05, ‡‡ p < 0.01: significantly different between X-ray/ADSC (+/−) and X-ray/ADSC (+/+). Symbols represent each study group ( n = 6 mice/group): X-ray/ADSC (−/−) (●), X-ray/ADSC (+/−) (△), X-ray/ADSC (+/+) (▲).

Article Snippet: Antigen retrieval treatment was performed using 0.01 M of citrate buffer (pH 6.0) at 85 °C for 20 min. For immunohistochemistry, antigen retrieved specimens were immersed in 3% hydrogen peroxide for 15 min, washed with tris(hydroxymethyl)aminomethane-buffered saline (TBS), and incubated with Blocking One Histo (nacalai tesque, Inc., Kyoto, Japan) for 10 min. After washing with TBS-0.1% Tween-20 (TBS-T), they were incubated 1 h at room temperature with rabbit polyclonal anti-mouse LYVE-1 antibody (2 μg/mL; DP3513P, OriGene Technologies Inc., Rockville, MD, USA).

Techniques: Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice

doi: 10.3390/ijms21113885

Figure Lengend Snippet: Mean lymphatic vessel areas with lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) immunoreactivity (mean ×10 3 pixel ± SE).

Article Snippet: Antigen retrieval treatment was performed using 0.01 M of citrate buffer (pH 6.0) at 85 °C for 20 min. For immunohistochemistry, antigen retrieved specimens were immersed in 3% hydrogen peroxide for 15 min, washed with tris(hydroxymethyl)aminomethane-buffered saline (TBS), and incubated with Blocking One Histo (nacalai tesque, Inc., Kyoto, Japan) for 10 min. After washing with TBS-0.1% Tween-20 (TBS-T), they were incubated 1 h at room temperature with rabbit polyclonal anti-mouse LYVE-1 antibody (2 μg/mL; DP3513P, OriGene Technologies Inc., Rockville, MD, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice

doi: 10.3390/ijms21113885

Figure Lengend Snippet: Ratios of proliferative lymphatic vessels with LYVE-1 and proliferating cell nuclear antigen (PCNA) immunoreactivity. ( A ) Representative images of immunofluorescence using anti-LYVE-1 (green) and anti-PCNA (red) antibody at day 8. Arrow heads: LYVE-1 and PCNA double-positive lymphatic endothelial cells. Scale bars (magnification): 50 µm (200×). ( B ) Ratio of proliferative lymphatic vessels (mean ± SE). Results of multiple comparisons within the same day groups are indicated. ** p < 0.01: significantly different between X-ray/ADSC (−/−) and X-ray/ADSC (+/−). †† p < 0.01: significantly different between X-ray/ADSC (−/−) and X-ray/ADSC (+/+). ‡‡ p < 0.01: significantly different between X-ray/ADSC (+/−) and X-ray/ADSC (+/+). Symbols represent each study group ( n = 3–6 mice/group): X-ray/ADSC (−/−) (●), X-ray/ADSC (+/−) (△), X-ray/ADSC (+/+) (▲).

Article Snippet: Antigen retrieval treatment was performed using 0.01 M of citrate buffer (pH 6.0) at 85 °C for 20 min. For immunohistochemistry, antigen retrieved specimens were immersed in 3% hydrogen peroxide for 15 min, washed with tris(hydroxymethyl)aminomethane-buffered saline (TBS), and incubated with Blocking One Histo (nacalai tesque, Inc., Kyoto, Japan) for 10 min. After washing with TBS-0.1% Tween-20 (TBS-T), they were incubated 1 h at room temperature with rabbit polyclonal anti-mouse LYVE-1 antibody (2 μg/mL; DP3513P, OriGene Technologies Inc., Rockville, MD, USA).

Techniques: Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice

doi: 10.3390/ijms21113885

Figure Lengend Snippet: Observation of scanning electron microscopy (SEM) in the X-ray/ADSC (+/+) groups. ( A , B ) Overlaid images of serial sections visualized by LYVE-1 immunohistochemistry and SEM observation. Rectangular shapes on (a) and (b) indicate position of high-magnification images (c–e). ( A ): Day 8, ( B ): Day 14. Scale bars (magnification); ( A -a) 200 µm (40×), ( A -b) 500 µm (37×), ( A -c) 20 µm (400×), ( A -d) 20 µm (900×), ( A -e) 10 µm (2000×), ( B -a) 200 µm (40×), ( B -b) 500 µm (35×), ( B -c) 50 µm (200×), ( B -d) 50 µm (300×), ( B -e) 10 µm (1200×).

Article Snippet: Antigen retrieval treatment was performed using 0.01 M of citrate buffer (pH 6.0) at 85 °C for 20 min. For immunohistochemistry, antigen retrieved specimens were immersed in 3% hydrogen peroxide for 15 min, washed with tris(hydroxymethyl)aminomethane-buffered saline (TBS), and incubated with Blocking One Histo (nacalai tesque, Inc., Kyoto, Japan) for 10 min. After washing with TBS-0.1% Tween-20 (TBS-T), they were incubated 1 h at room temperature with rabbit polyclonal anti-mouse LYVE-1 antibody (2 μg/mL; DP3513P, OriGene Technologies Inc., Rockville, MD, USA).

Techniques: Electron Microscopy, Immunohistochemistry